Induced pluripotent stem cells derived from one 70-years-old male donor with the APOE-ε4/ε4 alleles

Jin Wang; Xin Liu; Xiaofeng Du; Zhenhuan Ma; Boxin Liu; Ruiyun Guo; Baofeng Feng; Jun Ma; Huixian Cui

Stem Cell Research (May 2021)

Induced pluripotent stem cells derived from one 70-years-old male donor with the APOE-ε4/ε4 alleles

Jin Wang,
Xin Liu,
Xiaofeng Du,
Zhenhuan Ma,
Boxin Liu,
Ruiyun Guo,
Baofeng Feng,
Jun Ma,
Huixian Cui

Affiliations

Jin Wang: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; The Second Hospital of Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xin Liu: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xiaofeng Du: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Zhenhuan Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; The Second Hospital of Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Boxin Liu: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Ruiyun Guo: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Baofeng Feng: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Jun Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding authors at: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China. Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China.
Huixian Cui: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding authors at: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China. Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China.

Journal volume & issue: Vol. 53
p. 102395

Abstract

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Apolipoprotein E (ApoE) is a lipid-binding protein with ε2, ε3, and ε4 allelic variants in human. The ε4 isoform (ApoE4) is the strongest genetic risk factor for the late-onset form of Alzheimer’s disease (AD), and is also associated with multiple neurological disorders, multiple sclerosis, and cerebrovascular disease. Here, induced pluripotent stem cells were derived from the peripheral blood mononuclear cells of a 70-year-old male donor with APOE-ε4/ε4 alleles background to explore pathogenesis and screen potential treatment methods in neurodegenerative diseases. In the newly-developed induced pluripotent stem cell line, the pluripotent markers were well expressed. In addition, the generated cells displayed a normal karyotype and have differentiation potential.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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