Quantitative gravimetric analysis of bovine semen lipids by thin-layer chromatography

Abstract

The quantitative thin-layer chromatography technique described has the advantage inherent in TLC of speed plus great separating power. By increasing the thickness of the layer, lengthening the plate, and using two consecutive solvent systems on each plate, the procedure can successfully manage larger quantities of lipid, yet produce excellent separations. The technique affords advantages in preparative analysis.