TRIM72 inhibits cell migration and epithelial-mesenchymal transition by attenuating FAK/akt signaling in colorectal cancer
Oluwasijibomi Damola Faleti,
Yibing Gong,
Jingyi Long,
Qingshuang Luo,
Haiqi Tan,
Simin Deng,
Lizhen Qiu,
Xiaoming Lyu,
Jinke Yao,
Gongfa Wu
Affiliations
Oluwasijibomi Damola Faleti
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University. HKSAR, 999000, China
Yibing Gong
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China
Jingyi Long
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China
Qingshuang Luo
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China
Haiqi Tan
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China
Simin Deng
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China
Lizhen Qiu
Health Management Center, The Fourth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511300, China
Xiaoming Lyu
Department of Laboratory Medicine, The Third Affiliated Hospital of Southern Medical University. Guangzhou, Guangdong, 510630, China; Corresponding author. No. 183, West Zhongshan Avenue, Tianhe District, Guangzhou, China.
Jinke Yao
Department of general surgery, The Fourth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511300, China; Corresponding author. No. 1 Guangming East Road, Zengcheng District, Guangzhou, China.
Gongfa Wu
Department of pathology, The Fourth Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 511300, China; Corresponding author. No. 1 Guangming East Road, Zengcheng District, Guangzhou, China.
TRIM72 (MG53), a membrane repair protein with E3-ligase activity, plays a crucial role in colorectal cancer (CRC). This study examined TRIM72 expression in primary CRC tumors and paired liver metastases using RT-PCR. Findings revealed significantly lower TRIM72 levels in liver metastases compared to primary tumors (p < 0.001). Aberrant TRIM72 expression correlated with lymph node metastasis and advanced clinical stages. Overexpression of TRIM72 inhibited CRC cell migration, intravasation, and EMT in vitro and in vivo, while TRIM72 knockout increased migration and invasion. TRIM72 interacted with Focal Adhesion Kinase (FAK), implicating the FAK/Akt signaling axis in colon cancer spread. Lower TRIM72 levels were associated with reduced survival rates, highlighting its potential as a prognostic marker and therapeutic target in CRC.
