Generation and characterization of the LINC01405 knockout human embryonic stem cell line

Yaping Xu; Hongchun Wu; Jingxiu Jiang; Lingqun Ye; Kaili Hao; Kunjun Han; Shijun Hu; Wei Lei; Zhikun Guo

Stem Cell Research (Feb 2025)

Generation and characterization of the LINC01405 knockout human embryonic stem cell line

Yaping Xu,
Hongchun Wu,
Jingxiu Jiang,
Lingqun Ye,
Kaili Hao,
Kunjun Han,
Shijun Hu,
Wei Lei,
Zhikun Guo

Affiliations

Yaping Xu: The 7th People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan, China
Hongchun Wu: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Jingxiu Jiang: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Lingqun Ye: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Kaili Hao: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Kunjun Han: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Shijun Hu: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China
Wei Lei: Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China; Corresponding authors.
Zhikun Guo: The 7th People’s Hospital of Zhengzhou, Zhengzhou 450000, Henan, China; Corresponding authors.

Journal volume & issue: Vol. 82
p. 103619

Abstract

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Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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