Isolation of Primary Leydig Cells from Murine Testis
Jinlian Liang,
Yan Tang,
Hanhao Li,
Jiaxin Mei,
Zhen Cao,
Huan Xia,
Rufei Huang,
Yan Yang,
Yadong Huang
Affiliations
Jinlian Liang
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Yan Tang
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Hanhao Li
Department of Pharmacology, Jinan University, Guangzhou, China
Jiaxin Mei
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Zhen Cao
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Huan Xia
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Rufei Huang
Department of Pharmacology, Jinan University, Guangzhou, China
Yan Yang
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
Yadong Huang
Department of Cell Biology & Guangdong Provincial Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, ChinaDepartment of Pharmacology, Jinan University, Guangzhou, China
In males, Leydig cells are the primary source of testosterone, which is necessary for testis development, masculinization, and spermatogenesis. Leydig cells are a valuable cellular model for basic research; thus, it is important to develop an improved method for isolation and purification of Leydig cells from testes. The available methods for Leydig cell isolation have some drawbacks, including the need for sophisticated instruments, high cost, tediousness, and time consumption. Here, we describe an improved protocol for isolation of primary Leydig cells from testicular tissue by digestion with collagenase IV.
