Determination of abietic acid compounds in poultry by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry coupled with dispersive solid phase extraction

Abstract

Objective： An ultra performance liquid chromatography-quadrupole/time-of-flight mass spectrometry （UPLC-Q-TOF/MS） method was established for the determination of abietic acids in poultry. Methods： The samples were extracted with acetonitrile, then salted out and stratified. The supernatant was purified by C18 solid phase extractant and finally determined by UPLC-Q-TOF/MS in ESI negative mode. Results： Abietic acid compounds exhibited a good linear relationship in the concentration range of 10~500 μg/L with good correlation coefficient R2＞0.998. The average recoveries of three abietic acid compounds were experiments, the average recovery rate was 87.8%~98.5% with relative standard deviations （RSDs, n=6） of 1.99%~6.71%, at the spiked level of 20, 40, 200 μg/kg. The limits of detection （LODs） were 10 μg/kg and the method limits of quantification （LOQs） were 20 μg/kg. Conclusion： This method was convenient operation, rapid, high recovery and sensitivity and good precision that could meet the determination requirements of three abietic acids in poultry.

Keywords

• dispersive solid phase extraction
• uplc-q-tof/ms
• poultry
• abietic acid