Cancer Management and Research (Sep 2020)

LncRNA MCM3AP-AS1 Upregulates CDK4 by Sponging miR-545 to Suppress G1 Arrest in Colorectal Cancer

  • Ma X,
  • Luo J,
  • Zhang Y,
  • Sun D,
  • Lin Y

Journal volume & issue
Vol. Volume 12
pp. 8117 – 8124

Abstract

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Xudong Ma,1 Jing Luo,1 Yi Zhang,2 Dali Sun,1 Yueying Lin1 1Gastrointestinal Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming City, Yunnan Province 650031, People’s Republic of China; 2The General Surgery Department, The Teng-Chong People’s Hospital, Tengchong City, Yunan Province, 679100, People’s Republic of ChinaCorrespondence: Yueying Lin Email [email protected]: This study aimed to investigate the role of lncRNA MCM3AP-AS1 in colorectal cancer (CRC).Methods: Paired tumor and non-tumor tissues were collected from 60 CRC patients. Expression of MCM3AP-AS1 was determined by RT-qPCR. Overexpression of MCM3AP-AS1, miR-545, and CDK4 in CRC cells was achieved to explore the interactions between them. Cell cycle assay was performed to analyze the roles of MCM3AP-AS1, miR-545, and CDK4 in regulating the cell cycle progression of CRC cells.Results: We found that MCM3AP-AS1 was upregulated in CRC and its high expression levels predicted poor survival of CRC patients. MCM3AP-AS1 is predicted to interact with miR-545. In CRC cells, overexpression of MCM3AP-AS1 and miR-545 was achieved, while their overexpression did not affect the expression of each other. Instead, overexpression of MCM3AP-AS1 led to the increased expression levels of CDK4, which is a downstream target of miR-545. Cell cycle analysis showed that overexpression of MCM3AP-AS1 and CDK4 suppressed G1 arrest induced by miR-545. In addition, overexpression of MCM3AP-AS1 reduced the enhancing effects of overexpressing miR-545 on cell cycle progression.Conclusion: MCM3AP-AS1 may upregulate CDK4 by sponging miR-545 to induce G1 arrest in CRC cells.Keywords: MCM3AP-AS1, colorectal cancer, miR-545, CDK4, G1 arrest

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