IJCM_265A: Cellular Consequences of Fruit Juice Metallurgy: A Closer Look at Heavy Metal Influence

Abstract

Background: The leaching of heavy metals from packaging materials into packed juices can have several adverse effects on the quality and safety of the products and potentially pose health risks to consumers. Objective: The research aimed to investigate the cytotoxic effects of heavy metals specifically vanadium, cadmium, arsenic, and mercury, which are commonly found in packaging materials of marketable fruit juices which are leached into juices itself (orange and mixed fruit juices stored in cans, leak- proof bags, and tetraPaks). Methodology: The study utilized cell lines, namely HepG2 (human liver cancer) and HeK293 (human kidney), to assess the impact of these heavy metals on cellular viability, DNA damage, mitochondrial function, and cell cycle progression. Flow cytometry was used to analyse cell cycle alterations. Results: The MTT assay showcased dose-dependent decrease in cell viability with increasing concentrations of heavy metals. Annexin V Assay determined V, Hg and As had necrotic death pattern while Cd had apoptotic death pattern for both the cell lines in presence of heavy metal digests. Cells treated with heavy metals, and the resulting DNA damage observed to be high after quantified using comet tail length, olive tail movement, and tail moment measurements. Mitochondrial function assays in the presence of heavy metals revealed that increased mitochondrial membrane potential, mitochondrial mass, and reactive oxygen species production. ROS generation assessed to understand the impact of heavy metals on cellular health and oxidative stress. Conclusion: This study shed light on the potential cytotoxic effects of heavy metals found in fruit juices. These findings contribute to our understanding of the potential risks associated with heavy metal exposure from consumer products and emphasize the importance of regulatory measures to ensure food safety and human health.

Keywords

• leaching
• hepg2
• hek293
• heavy metal digests