Communications Biology (Mar 2022)

Non-invasive human skin transcriptome analysis using mRNA in skin surface lipids

  • Takayoshi Inoue,
  • Tetsuya Kuwano,
  • Yuya Uehara,
  • Michiko Yano,
  • Naoki Oya,
  • Naoto Takada,
  • Shodai Tanaka,
  • Yui Ueda,
  • Akira Hachiya,
  • Yoshito Takahashi,
  • Noriyasu Ota,
  • Takatoshi Murase

DOI
https://doi.org/10.1038/s42003-022-03154-w
Journal volume & issue
Vol. 5, no. 1
pp. 1 – 13

Abstract

Read online

Inoue et al develop a non-invasive method of analyzing human skin mRNA using RNA in skin surface lipids collected with oil-blotting films. The authors outline the validation of this methodology and describe an application to determine transcriptome in skin surface lipids in patients with atopic dermatitis versus healthy skin.