Cytotoxic, Anti-Migration, and Anti-Invasion Activities on Breast Cancer Cells of Angucycline Glycosides Isolated from a Marine-Derived <i>Streptomyces</i> sp.
Xin-Ying Qu,
Jin-Wei Ren,
Ai-Hong Peng,
Shi-Qi Lin,
Dan-Dan Lu,
Qian-Qian Du,
Ling Liu,
Xia Li,
Er-Wei Li,
Wei-Dong Xie
Affiliations
Xin-Ying Qu
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Jin-Wei Ren
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Ai-Hong Peng
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Shi-Qi Lin
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Dan-Dan Lu
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Qian-Qian Du
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Ling Liu
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Xia Li
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Er-Wei Li
State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
Wei-Dong Xie
College of Marine Science, Shandong University at Weihai, Weihai 264209, China
Four angucycline glycosides were previously characterized from marine-derived Streptomyces sp. OC1610.4. Further investigation of this strain cultured on different fermentation media from that used previously resulted in the isolation of two new angucycline glycosides, vineomycins E and F (1−2), and five known homologues, grincamycin L (3), vineomycinone B2 (4), fridamycin D (5), moromycin B (7), and saquayamycin B1 (8). Vineomycin F (2) contains an unusual ring-cleavage deoxy sugar. All the angucycline glycosides isolated from Streptomyces sp. OC1610.4 were evaluated for their cytotoxic activity against breast cancer cells MCF-7, MDA-MB-231, and BT-474. Moromycin B (7), saquayamycin B1 (8), and saquayamycin B (9) displayed potent anti-proliferation against the tested cell lines, with IC50 values ranging from 0.16 to 0.67 μM. Saquayamycin B (9) inhibited the migration and invasion of MDA-MB-231 cells in a dose-dependent manner, as detected by Transwell and wound-healing assays.
