Calibrating-free Portable Magnetoelastic Device for Accurate Detection of Anti-CSFV-E2 in Porcine Serum

Xing GUO; Man LUO; Jianru HOU; Yuchao LI; Shuhua WANG; Xiaoliang CHENG; Aoqun JIAN; Zhongyun YUAN; Shengbo SANG

doi:10.16355/j.tyut.1007-9432.2023.05.018

Taiyuan Ligong Daxue xuebao (Sep 2023)

Calibrating-free Portable Magnetoelastic Device for Accurate Detection of Anti-CSFV-E2 in Porcine Serum

Xing GUO,
Man LUO,
Jianru HOU,
Yuchao LI,
Shuhua WANG,
Xiaoliang CHENG,
Aoqun JIAN,
Zhongyun YUAN,
Shengbo SANG

Affiliations

Xing GUO: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Man LUO: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Jianru HOU: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Yuchao LI: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Shuhua WANG: College of Animal Science, Shanxi Agricultural University, Taigu 030801, China
Xiaoliang CHENG: College of Animal Medicine, Shanxi Agricultural University, Taigu 030801, China
Aoqun JIAN: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Zhongyun YUAN: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Shengbo SANG: Shanxi Key Laboratory of Micro Nano Sensors & Artificial Intelligence Perception, College of Electronic Information and Optical Engineering, Taiyuan University of Technology, Taiyuan 030024, China

DOI: https://doi.org/10.16355/j.tyut.1007-9432.2023.05.018
Journal volume & issue: Vol. 54, no. 5
pp. 891 – 897

Abstract

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Purposes Error calibration between equipment manufacturing has become a practical challenge for various inspection methods. Methods In this paper, a portable magnetoelastic (ME) biodetection device is proposed for calibration-free detection of classical swine fever virus E2 antibody (anti-CSFV E2), on the basis of magnetostrictive effect, by monitoring the resonance frequency shift caused by anti-CSFV E2 to react to the concentration of analytes. The device consists of two parts, namely the magnetoelastic test strip and the intelligent magnetoelastic resonance frequency detector. The test strip is very small, and the external dimension is about 1/20 of the size of the ELISA kit. By using the ELISA kit as a control, the accuracy of the portable ME device is verified by detecting the same concentration of pig serum samples. Findings The results show that the detection results of the calibration-free portable ME device and the ELISA kit have good consistency, and the accuracy rate is 98%. The experimental results show that the sensitivity is 7.20 Hz/(μg/mL) and the linear range is 0.1~100 μg/mL, and the device can quickly and accurately detect anti-CSFV E2 without calibration, with good specificity and stability. Conclusions The calibration-free portable ME unit provides an effective method for the diagnosis of swine fever and the monitoring of the effectiveness of anti-swine fever vaccination.

Published in Taiyuan Ligong Daxue xuebao

ISSN: 1007-9432 (Print)
Publisher: Editorial Office of Journal of Taiyuan University of Technology
Country of publisher: China
LCC subjects: Technology: Chemical technology: Chemical engineering; Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://tyutjournal.tyut.edu.cn/english.html

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