Long-Term Recording of LTP in Cultured Hippocampal Slices

Ken  Shimono; Michel  Baudry; Lam  Ho; Makoto  Taketani; Gary  Lynch

doi:10.1155/NP.2002.249

Neural Plasticity (Jan 2002)

Long-Term Recording of LTP in Cultured Hippocampal Slices

Ken Shimono,
Michel Baudry,
Lam Ho,
Makoto Taketani,
Gary Lynch

Affiliations

Ken Shimono: Tensor Biosciences, Irvine 92612, CA, USA
Michel Baudry: Dept. Psychiatry and Human Behavior, University of California, 101 Theory Dr., suite 250, Irvine 92697, CA, USA
Lam Ho: Tensor Biosciences, Irvine 92612, CA, USA
Makoto Taketani: Tensor Biosciences, Irvine 92612, CA, USA
Gary Lynch: Dept. Psychiatry and Human Behavior, University of California, 101 Theory Dr., suite 250, Irvine 92697, CA, USA

DOI: https://doi.org/10.1155/NP.2002.249
Journal volume & issue: Vol. 9, no. 4
pp. 249 – 254

Abstract

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Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was completely and reversibly blocked by an antagonist of the NMDA receptor, APV. Our results suggest the possibility of using chronic recording in hippocampal slices cultured on multi-electrode arrays to study the mechanisms underlying LTP maintenance and stabilization.

Published in Neural Plasticity

ISSN: 2090-5904 (Print); 1687-5443 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.hindawi.com/journals/np/

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