Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution

Kaiming Zhang; Shanshan Li; Kalli Kappel; Grigore Pintilie; Zhaoming Su; Tung-Chung Mou; Michael F. Schmid; Rhiju Das; Wah Chiu

doi:10.1038/s41467-019-13494-7

Nature Communications (Dec 2019)

Cryo-EM structure of a 40 kDa SAM-IV riboswitch RNA at 3.7 Å resolution

Kaiming Zhang,
Shanshan Li,
Kalli Kappel,
Grigore Pintilie,
Zhaoming Su,
Tung-Chung Mou,
Michael F. Schmid,
Rhiju Das,
Wah Chiu

Affiliations

Kaiming Zhang: Department of Bioengineering, and James H. Clark Center, Stanford University
Shanshan Li: Department of Bioengineering, and James H. Clark Center, Stanford University
Kalli Kappel: Biophysics Program, Stanford University
Grigore Pintilie: Department of Bioengineering, and James H. Clark Center, Stanford University
Zhaoming Su: Department of Bioengineering, and James H. Clark Center, Stanford University
Tung-Chung Mou: Center for Biomolecular Structure and Dynamics, University of Montana
Michael F. Schmid: Division of CryoEM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Stanford University
Rhiju Das: Department of Biochemistry, Stanford University
Wah Chiu: Department of Bioengineering, and James H. Clark Center, Stanford University

DOI: https://doi.org/10.1038/s41467-019-13494-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 6

Abstract

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The conformational heterogeneity of RNA molecules makes their structure determination by X-ray crystallography and NMR challenging. Here the authors show that RNA structures can be solved by cryo-EM and present the structures of a 40 kDa SAM-IV riboswitch in the apo form and bound to its ligand S-adenosylmethionine.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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