Frontiers in Microbiology (Mar 2022)
Herpes Simplex Virus 1 Induces Microglia Gasdermin D-Dependent Pyroptosis Through Activating the NLR Family Pyrin Domain Containing 3 Inflammasome
- Xiao Hu,
- Xiao Hu,
- Xiao Hu,
- Xiao Hu,
- Xiao Hu,
- Xiao Hu,
- Qiongzhen Zeng,
- Qiongzhen Zeng,
- Qiongzhen Zeng,
- Qiongzhen Zeng,
- Qiongzhen Zeng,
- Ji Xiao,
- Ji Xiao,
- Ji Xiao,
- Ji Xiao,
- Ji Xiao,
- Shurong Qin,
- Shurong Qin,
- Shurong Qin,
- Shurong Qin,
- Shurong Qin,
- Shurong Qin,
- Yuan Wang,
- Yuan Wang,
- Yuan Wang,
- Yuan Wang,
- Yuan Wang,
- Tianhao Shan,
- Tianhao Shan,
- Tianhao Shan,
- Tianhao Shan,
- Tianhao Shan,
- Di Hu,
- Di Hu,
- Di Hu,
- Di Hu,
- Di Hu,
- Di Hu,
- Yexuan Zhu,
- Yexuan Zhu,
- Yexuan Zhu,
- Yexuan Zhu,
- Yexuan Zhu,
- Kaisheng Liu,
- Kai Zheng,
- Yifei Wang,
- Yifei Wang,
- Yifei Wang,
- Yifei Wang,
- Yifei Wang,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren,
- Zhe Ren
Affiliations
- Xiao Hu
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Xiao Hu
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Xiao Hu
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Xiao Hu
- College of Pharmacy, Jinan University, Guangzhou, China
- Xiao Hu
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Xiao Hu
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Qiongzhen Zeng
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Qiongzhen Zeng
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Qiongzhen Zeng
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Qiongzhen Zeng
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Qiongzhen Zeng
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Ji Xiao
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Ji Xiao
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Ji Xiao
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Ji Xiao
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Ji Xiao
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Shurong Qin
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Shurong Qin
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Shurong Qin
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Shurong Qin
- College of Pharmacy, Jinan University, Guangzhou, China
- Shurong Qin
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Shurong Qin
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Yuan Wang
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Yuan Wang
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Yuan Wang
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Yuan Wang
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Yuan Wang
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Tianhao Shan
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Tianhao Shan
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Tianhao Shan
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Tianhao Shan
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Tianhao Shan
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Di Hu
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Di Hu
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Di Hu
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Di Hu
- College of Pharmacy, Jinan University, Guangzhou, China
- Di Hu
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Di Hu
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Yexuan Zhu
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Yexuan Zhu
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Yexuan Zhu
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Yexuan Zhu
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Yexuan Zhu
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Kaisheng Liu
- Shenzhen People’s Hospital (The Second Clinical Medical College, Jinan University, The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen, China
- Kai Zheng
- School of Pharmaceutical Sciences, Health Science Center, Shenzhen University, Shenzhen, China
- Yifei Wang
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Yifei Wang
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Yifei Wang
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Yifei Wang
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Yifei Wang
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- Zhe Ren
- Guangzhou Jinan Biomedical Research and Development Center, College of Life Science and Technology, Institute of Biomedicine, Jinan University, Guangzhou, China
- Zhe Ren
- The Key Laboratory of Virology of Guangdong, Jinan University, Guangzhou, China
- Zhe Ren
- Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China
- Zhe Ren
- Guangdong Province Key Laboratory of Bioengineering Medicine, Jinan University, Guangzhou, China
- Zhe Ren
- National Engineering Research Center of Genetic Medicine, Jinan University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fmicb.2022.838808
- Journal volume & issue
-
Vol. 13
Abstract
Herpes simplex virus type 1 (HSV-1) is a highly prevalent virus in humans and causes severe forms of inflammation, such as herpes simplex encephalitis (HSE). Pyroptosis is a new inflammatory cell death triggered by inflammasome and cysteine-requiring aspartate protease-1 (caspase-1) activation. Nonetheless, HSV-1 induces encephalitis, and cell death mechanisms are not understood. In this study, we confirmed for the first time that the DNA virus HSV-1 triggers Gasdermin D-dependent pyroptosis by activating NLR family pyrin domain containing 3 (NLRP3) inflammasomes in mouse microglia, leading to mature IL-1β production and active caspase-1 (p10) release. Inhibition of microglial NLRP3 inflammasome activation suppressed HSV-1-induced Gasdermin D-dependent pyroptosis. In addition, NLRP3 and IL-1β expression levels were significantly increased in the mouse model of herpes simplex encephalitis compared with normal mice without viral infection. Collectively, our data revealed that the activation of inflammasomes and GSDMD-dependent pyroptosis is the mechanism of HSV-1 inducing inflammation and provides treatment targets for viral inflammation.
Keywords
- pyroptosis
- inflammation
- NLRP3 inflammasome
- Gasdermin D
- Herpes simplex virus type 1
- herpes simplex virus encephalitis
