Veterinary Medicine: Research and Reports (Apr 2022)
Isolation and Molecular Detection of Pasteurellosis from Pneumonic Sheep in Selected Areas of Amhara Region, Ethiopia: An Implication for Designing Effective Ovine Pasteurellosis Vaccine
Abstract
Aragaw Ebabu Akane,1 Gashaw Alemu,2 Kidest Tesfaye,2 Destaw Asfaw Ali,2 Takele Abayneh,3 Ambaye Kenubih,2 Mebrat Ejo,4 Anmaw Shite Abat,2 Bemrew Admassu,4 Saddam Mohammed Ibrahim2 1Lalibela Town Administration Agricultural Office, Lalibela, Ethiopia; 2Department of Veterinary Paraclinical Studies, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, Ethiopia; 3National Veterinary Institute, Bishoftu, Ethiopia; 4Department of Veterinary Biomedical Sciences, College of Veterinary Medicine and Animal Sciences, University of Gondar, Gondar, EthiopiaCorrespondence: Saddam Mohammed Ibrahim, Email [email protected]: Pneumonic pasteurellosis mainly caused by bacterial species of Mannheimia, Pasteurella, and Bibersteinia causes a significant financial loss to the sheep production sector through reduced productivity and high mortality. There is a dearth of information on the major agents involved in the disease in the Amhara region, Ethiopia. Therefore, the aim of this study was to isolate and molecularly confirm Mannheimia, Pasteurella, and Bibersteinia from nasal swabs of sheep suspected of pneumonic pasteurellosis in selected areas of the Amhara region.Methods: Isolation and phenotypic characterization were performed using microbiological and biochemical testing according to standard methods. Molecular confirmation of isolates was done through amplification of virulence associated genes, PHSAA and Rpt2, of Mannheimia hemolyticausing multiplex PCR.Results: Accordingly, 46 out of 141 (32.62%) samples were presumably identified as M. hemolytica with no Pasteurella multocida and Bibersteinia trehalosi. Seven (n=7) out of the 46 isolates tested positive for either of the two virulence genes.Discussion and conclusion: The finding of this study is indicative that M. hemolytica is the main bacteria linked with pneumonic pasteurellosis in the study area which suggests the need to develop a polyvalent vaccine including strains of M. hemolytica or its antigenic determinants. However, the role of other bacterial, viral, and parasitic agents in the cases investigated should also be considered.Keywords: Bibersteinia trehalosi, isolation, Mannheimia hemolytica, Pasteurella multocida, PHSSA, pneumonic pasteurellosis, Rpt2, sheep
