An electrochemiluminescence resonance energy transfer biosensor for the detection of circulating tumor DNA from blood plasma
Xidong Yang,
Meiyan Liao,
Hanfei Zhang,
JinBo Gong,
Fan Yang,
Mengying Xu,
Pier-Luc Tremblay,
Tian Zhang
Affiliations
Xidong Yang
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; Shaoxing Institute for Advanced Research, Wuhan University of Technology, Shaoxing 312300, PR China
Meiyan Liao
Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Hanfei Zhang
Department of Radiology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
JinBo Gong
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China
Fan Yang
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China
Mengying Xu
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; Shaoxing Institute for Advanced Research, Wuhan University of Technology, Shaoxing 312300, PR China
Pier-Luc Tremblay
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; Shaoxing Institute for Advanced Research, Wuhan University of Technology, Shaoxing 312300, PR China; State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China; Corresponding author
Tian Zhang
School of Chemistry, Chemical Engineering, and Life Science, Wuhan University of Technology, Wuhan 430070, PR China; Shaoxing Institute for Advanced Research, Wuhan University of Technology, Shaoxing 312300, PR China; State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, Wuhan 430070, PR China; School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, PR China; Corresponding author
Summary: A liquid biopsy is a noninvasive approach for detecting double-stranded circulating tumor DNA (ctDNA) of 90–320 nucleotides in blood plasma from patients with cancer. Most techniques employed for ctDNA detection are time consuming and require expensive DNA purification kits. Electrochemiluminescence resonance energy transfer (ECL-RET) biosensors exhibit high sensitivity, a wide response range, and are promising for straightforward sensing applications. Until now, ECL-RET biosensors have been designed for sensing short single-stranded oligonucleotides of less than 45 nucleotides. In this work, an ECL-RET biosensor comprising graphitic carbon nitride quantum dots was assessed for the amplification-free detection in the blood plasma of DNA molecules coding for the EGFR L858R mutation, which is associated with non-small-cell lung cancer. Following a low-cost pre-treatment, the highly specific ECL-RET biosensor quantified double-stranded EGFR L858R DNA of 159 nucleotides diluted into the blood within a linear range of 0.01 fM to 1 pM, demonstrating its potential for noninvasive biopsies.
