Integrative analysis of transcriptomics and metabolomics to reveal the melanogenesis pathway of muscle and related meat characters in Wuliangshan black-boned chickens
Tengfei Dou,
Shixiong Yan,
Lixian Liu,
Kun Wang,
Zonghui Jian,
Zhiqiang Xu,
Jingying Zhao,
Qiuting Wang,
Shuai Sun,
Mir Zulqarnain Talpur,
Xiaohua Duan,
Dahai Gu,
Yang He,
Yanli Du,
Alsoufi Mohammed Abdulwahid,
Qihua Li,
Hua Rong,
Weina Cao,
Zhengchang Su,
Guiping Zhao,
Ranran Liu,
Sumei Zhao,
Ying Huang,
Marinus F. W. te Pas,
Changrong Ge,
Junjing Jia
Affiliations
Tengfei Dou
Faculty of Animal Science and Technology, Yunnan Agricultural University
Shixiong Yan
Faculty of Animal Science and Technology, Yunnan Agricultural University
Lixian Liu
Faculty of Animal Science and Technology, Yunnan Agricultural University
Kun Wang
Faculty of Animal Science and Technology, Yunnan Agricultural University
Zonghui Jian
Faculty of Animal Science and Technology, Yunnan Agricultural University
Zhiqiang Xu
Faculty of Animal Science and Technology, Yunnan Agricultural University
Jingying Zhao
Faculty of Animal Science and Technology, Yunnan Agricultural University
Qiuting Wang
Faculty of Animal Science and Technology, Yunnan Agricultural University
Shuai Sun
Faculty of Animal Science and Technology, Yunnan Agricultural University
Mir Zulqarnain Talpur
Faculty of Animal Science and Technology, Yunnan Agricultural University
Xiaohua Duan
Faculty of Animal Science and Technology, Yunnan Agricultural University
Dahai Gu
Faculty of Animal Science and Technology, Yunnan Agricultural University
Yang He
Faculty of Animal Science and Technology, Yunnan Agricultural University
Yanli Du
Faculty of Animal Science and Technology, Yunnan Agricultural University
Alsoufi Mohammed Abdulwahid
Faculty of Animal Science and Technology, Yunnan Agricultural University
Qihua Li
Faculty of Animal Science and Technology, Yunnan Agricultural University
Hua Rong
Faculty of Animal Science and Technology, Yunnan Agricultural University
Weina Cao
Faculty of Animal Science and Technology, Yunnan Agricultural University
Zhengchang Su
Department of Bioinformatics and Genomics, College of Computing and Informatics, the University of North Carolina at Charlotte
Guiping Zhao
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
Ranran Liu
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences
Sumei Zhao
Faculty of Animal Science and Technology, Yunnan Agricultural University
Ying Huang
Faculty of Animal Science and Technology, Yunnan Agricultural University
Marinus F. W. te Pas
Wageningen Livestock Research
Changrong Ge
Faculty of Animal Science and Technology, Yunnan Agricultural University
Junjing Jia
Faculty of Animal Science and Technology, Yunnan Agricultural University
Abstract Background Melanin is an important antioxidant in food and has been used in medicine and cosmetology. Chicken meat with high melanin content from black-boned chickens have been considered a high nutritious food with potential medicinal properties. The molecular mechanism of melanogenesis of skeletal muscle in black-boned chickens remain poorly understood. This study investigated the biological gene-metabolite associations regulating the muscle melanogenesis pathways in Wuliangshan black-boned chickens with two normal boned chicken breeds as control. Results We identified 25 differentially expressed genes and 11 transcription factors in the melanogenesis pathways. High levels of the meat flavor compounds inosine monophosphate, hypoxanthine, lysophospholipid, hydroxyoctadecadienoic acid, and nicotinamide mononucleotide were found in Wuliangshan black-boned chickens. Conclusion Integrative analysis of transcriptomics and metabolomics revealed the dual physiological functions of the PDZK1 gene, involved in pigmentation and/or melanogenesis and regulating the phospholipid signaling processes in muscle of black boned chickens.
