Frontiers in Immunology (Nov 2024)
Unbiased immunome characterisation correlates with COVID-19 mRNA vaccine failure in immunocompromised adults
- Juan H-Vázquez,
- Paloma Cal-Sabater,
- Elisa Arribas-Rodríguez,
- Aida Fiz-López,
- Candido Perez-Segurado,
- Álvaro Martín-Muñoz,
- Ángel De Prado,
- Marina Perez Mazzali,
- Carolina G. de Castro,
- Alejandro G. del Hierro,
- Ignacio de la Fuente Graciani,
- Sonia Pérez González,
- Sara Gutiérrez,
- Pablo Tellería,
- Cristina Novoa,
- Silvia Rojo Rello,
- Antonio Garcia-Blesa,
- Rosa Sedano,
- Ana María Martínez García,
- Sonsoles Garcinuño Pérez,
- Marta Domínguez-Gil,
- Cristina Hernán García,
- Ma Mercedes Guerra,
- Eduardo Muñoz-Sánchez,
- Cristina Barragan-Pérez,
- Soraya Diez Morales,
- Oriana Casazza Donnarumma,
- Daniel Ramos Pollo,
- Natalia Santamarta Solla,
- Paula Ma Álvarez Manzanares,
- Sara Bravo,
- Cristina García Alonso,
- Luis Alberto Avendaño Fernández,
- Jenifer Gay Alonso,
- José A. Garrote,
- Eduardo Arranz,
- José María Eiros,
- Fernando Rescalvo Santiago,
- Carolina Quevedo Villegas,
- Eduardo Tamayo,
- Eduardo Tamayo,
- Antonio Orduña,
- Carlos Dueñas,
- María Jesús Peñarrubia,
- Sara Cuesta-Sancho,
- María Montoya,
- David Bernardo,
- David Bernardo
Affiliations
- Juan H-Vázquez
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Paloma Cal-Sabater
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Elisa Arribas-Rodríguez
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Aida Fiz-López
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Candido Perez-Segurado
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Álvaro Martín-Muñoz
- Flow Cytometry Facility, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Ángel De Prado
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Marina Perez Mazzali
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Carolina G. de Castro
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Alejandro G. del Hierro
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Ignacio de la Fuente Graciani
- Department of Hematology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Sonia Pérez González
- Department of Hematology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Sara Gutiérrez
- Internal Medicine Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Pablo Tellería
- Internal Medicine Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Cristina Novoa
- Internal Medicine Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Silvia Rojo Rello
- Microbiology and Inmunology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Antonio Garcia-Blesa
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Rosa Sedano
- Internal Medicine Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Ana María Martínez García
- Microbiology and Inmunology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Sonsoles Garcinuño Pérez
- Microbiology and Inmunology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Marta Domínguez-Gil
- Microbiology Department, Hospital Universitario Río Hortega, Valladolid, Spain
- Cristina Hernán García
- Servicio de Medicina Preventiva, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Ma Mercedes Guerra
- Servicio de Medicina Preventiva, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Eduardo Muñoz-Sánchez
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Cristina Barragan-Pérez
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Soraya Diez Morales
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Oriana Casazza Donnarumma
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Daniel Ramos Pollo
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Natalia Santamarta Solla
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Paula Ma Álvarez Manzanares
- Unidad de Cuidados Paliativos del Hospital Universitario Río Hortega, Valladolid, Spain
- Sara Bravo
- Department of Hematology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Cristina García Alonso
- Department of Hematology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Luis Alberto Avendaño Fernández
- Servicio de Prevención de Riesgos Laborales, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Jenifer Gay Alonso
- 0Residencia y Centro de Día ORPEA Valladolid, Valladolid, Spain
- José A. Garrote
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- Eduardo Arranz
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- José María Eiros
- Microbiology Department, Hospital Universitario Río Hortega, Valladolid, Spain
- Fernando Rescalvo Santiago
- Servicio de Prevención de Riesgos Laborales, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Carolina Quevedo Villegas
- Servicio de Prevención de Riesgos Laborales, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Eduardo Tamayo
- 1Unidad Investigación, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Eduardo Tamayo
- 2Centro de Investigaciones Biomédicas en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain
- Antonio Orduña
- Microbiology and Inmunology Unit, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Carlos Dueñas
- Internal Medicine Department, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- María Jesús Peñarrubia
- Department of Hematology, Hospital Clínico Universitario de Valladolid, Valladolid, Spain
- Sara Cuesta-Sancho
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- María Montoya
- 3Viral Immunology, Therapies and Vaccines Lab, Centro de Investigaciones Biológicas Margarita Salas (CIB), Madrid, Spain
- David Bernardo
- Mucosal Immunology Lab, Unit of Excellence Institute of Biomedicine and Molecular Genetics of Valladolid (IBGM, University of Valladolid-CSIC), Valladolid, Spain
- David Bernardo
- 2Centro de Investigaciones Biomédicas en Red de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain
- DOI
- https://doi.org/10.3389/fimmu.2024.1405217
- Journal volume & issue
-
Vol. 15
Abstract
IntroductionCoronavirus disease 2019 (COVID-19) affects the population unequally, with a greater impact on older and immunosuppressed people.MethodsHence, we performed a prospective experimental cohort study to characterise the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination in immune-compromised patients (older adults and oncohaematologic patients), compared with healthy counterparts, based on deep characterisation of the circulating immune cell subsets.Results and discussionWhile acquired humoral and cellular memory did not predict subsequent infection 18 months after full vaccination, spectral and computational cytometry revealed several subsets within the CD8+ T-cells, B-cells, natural killer (NK) cells, monocytes and TEMRA Tγδ cells that were differentially expressed in individuals who were subsequently infected and not infected not just following immunisation, but also prior to vaccination. Of note, we found up to seven clusters within the TEMRA Tγδ cell population, with some of them being expanded and others decreased in subsequently infected individuals. Moreover, some of these cellular clusters were also related to COVID-19-induced hospitalisation in oncohaematologic patients. Therefore, we have identified a cellular signature that even before vaccination is related to COVID-19 vulnerability as opposed to the acquisition of cellular and/or humoral memory following vaccination with SARS-CoV-2 messenger RNA (mRNA) vaccines.
Keywords
