Sensors (Feb 2014)

A Disposable Alkaline Phosphatase-Based Biosensor for Vanadium Chronoamperometric Determination

  • Ana Lorena Alvarado-Gámez,
  • María Asunción Alonso-Lomillo,
  • Olga Domínguez-Renedo,
  • María Julia Arcos-Martínez

DOI
https://doi.org/10.3390/s140203756
Journal volume & issue
Vol. 14, no. 2
pp. 3756 – 3767

Abstract

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A chronoamperometric method for vanadium ion determination, based on the inhibition of the enzyme alkaline phosphatase, is reported. Screen-printed carbon electrodes modified with gold nanoparticles were used as transducers for the immobilization of the enzyme. The enzymatic activity over 4-nitrophenyl phosphate sodium salt is affected by vanadium ions, which results in a decrease in the chronoamperometric current registered. The developed method has a detection limit of 0.39 ± 0.06 µM, a repeatability of 7.7% (n = 4) and a reproducibility of 8% (n = 3). A study of the possible interferences shows that the presence of Mo(VI), Cr(III), Ca(II) and W(VI), may affect vanadium determination at concentration higher than 1.0 mM. The method was successfully applied to the determination of vanadium in spiked tap water.

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