Callus culture and micropropagation of Eustoma grandiflorum Shinn.

Abstract

Regenerated plants and callus tissue were obtained by embryo culture and micropropagation of explants from shoots and leaves of Eustoma grandiflorum Shinn. (Gentianaceae), a medicinal plant. This species, which does not naturally occur in this country, but is found in the southwestern regions of North America, can be propagated in vitro in a relatively short time. Regenerated plants were obtained in amounts enabling extensive phytochemical analyses to be conducted.

Keywords

• in vitro
• callus culture
• micropropagation
• Eustoma
• Gentiancaceae