Using high-performance liquid chromatography with UV detector to quantify exhaled leukotriene B4 level in nonatopic adults

Abstract

This study aimed to evaluate the feasibility of the chemical method to analyze exhaled breath condensate (EBC) leukotriene B4 (LTB4) level in humans. High-performance liquid chromatography with a UV detector was applied to quantify the inflammatory biomarker. The LTB4 concentration in the concentrated pooled EBC samples was 1.19 ng/μL, and the average LTB4 concentration of each EBC sample was 15.38 ng/μL. This analytical technique was feasible to evaluate the levels of inflammatory mediators such as LTB4 in human EBCs without any complicated sample pretreatment processes.

Keywords

• exhaled breath condensate
• high-performance liquid chromatography
• leukotriene B4
• leukotrienes