The Scientific World Journal (Jan 2014)

A Lectin from Dioclea violacea Interacts with Midgut Surface of Lutzomyia migonei, Unlike Its Homologues, Cratylia floribunda Lectin and Canavalia gladiata Lectin

  • Juliana Montezuma Barbosa Monteiro Tínel,
  • Melina Fechine Costa Benevides,
  • Mércia Sindeaux Frutuoso,
  • Camila Farias Rocha,
  • Francisco Vassiliepe Sousa Arruda,
  • Mayron Alves Vasconcelos,
  • Francisco Nascimento Pereira-Junior,
  • João Batista Cajazeiras,
  • Kyria Santiago do Nascimento,
  • Jorge Luiz Martins,
  • Edson Holanda Teixeira,
  • Benildo Sousa Cavada,
  • Ricardo Pires dos Santos,
  • Margarida Maria Lima Pompeu

DOI
https://doi.org/10.1155/2014/239208
Journal volume & issue
Vol. 2014

Abstract

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Leishmaniasis is a vector-borne disease transmitted by phlebotomine sand fly. Susceptibility and refractoriness to Leishmania depend on the outcome of multiple interactions that take place within the sand fly gut. Promastigote attachment to sand fly midgut epithelium is essential to avoid being excreted together with the digested blood meal. Promastigote and gut sand fly surface glycans are important ligands in this attachment. The purpose of the present study was to evaluate the interaction of three lectins isolated from leguminous seeds (Diocleinae subtribe), D-glucose and D-mannose-binding, with glycans on Lutzomyia migonei midgut. To study this interaction the lectins were labeled with FITC and a fluorescence assay was performed. The results showed that only Dioclea violacea lectin (DVL) was able to interact with midgut glycans, unlike Cratylia floribunda lectin (CFL) and Canavalia gladiata lectin (CGL). Furthermore, when DVL was blocked with D-mannose the interaction was inhibited. Differences of spatial arrangement of residues and volume of carbohydrate recognition domain (CRD) may be the cause of the fine specificity of DVL for glycans in the surface on Lu. migonei midgut. The findings in this study showed the presence of glycans in the midgut with glucose/mannose residues in its composition and these residues may be important in interaction between Lu. migonei midgut and Leishmania.