Frontiers in Plant Science (May 2023)
Identification and validation of two major QTLs for spikelet number per spike in wheat (Triticum aestivum L.)
- Xiaoyu Yi,
- Xiaoyu Yi,
- Xiaoyu Yi,
- Yingtong Ye,
- Yingtong Ye,
- Yingtong Ye,
- Jinhui Wang,
- Jinhui Wang,
- Jinhui Wang,
- Zhen Li,
- Zhen Li,
- Zhen Li,
- Jiamin Li,
- Jiamin Li,
- Jiamin Li,
- Yuqi Chen,
- Guoyue Chen,
- Guoyue Chen,
- Jian Ma,
- Jian Ma,
- Zhien Pu,
- Zhien Pu,
- Zhien Pu,
- Yuanying Peng,
- Yuanying Peng,
- Pengfei Qi,
- Pengfei Qi,
- Yaxi Liu,
- Yaxi Liu,
- Qiantao Jiang,
- Qiantao Jiang,
- Jirui Wang,
- Jirui Wang,
- Yuming Wei,
- Yuming Wei,
- Youliang Zheng,
- Youliang Zheng,
- Wei Li,
- Wei Li,
- Wei Li
Affiliations
- Xiaoyu Yi
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Xiaoyu Yi
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Xiaoyu Yi
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yingtong Ye
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yingtong Ye
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Yingtong Ye
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jinhui Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jinhui Wang
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Jinhui Wang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Zhen Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Zhen Li
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Zhen Li
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jiamin Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jiamin Li
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Jiamin Li
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yuqi Chen
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Guoyue Chen
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jian Ma
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Zhien Pu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Zhien Pu
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Zhien Pu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yuanying Peng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yuanying Peng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Pengfei Qi
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Pengfei Qi
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Yaxi Liu
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yaxi Liu
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Qiantao Jiang
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Jirui Wang
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Jirui Wang
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Yuming Wei
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Yuming Wei
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Youliang Zheng
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Youliang Zheng
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- Wei Li
- State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, China
- Wei Li
- College of Agronomy, Sichuan Agricultural University, Chengdu, China
- Wei Li
- Triticeae Research Institute, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fpls.2023.1144486
- Journal volume & issue
-
Vol. 14
Abstract
The total number of spikelets (TSPN) and the number of fertile spikelets (FSPN) affect the final number of grains per spikelet in wheat. This study constructed a high-density genetic map using 55K single nucleotide polymorphism (SNP) arrays from a population of 152 recombinant inbred lines (RIL) from crossing the wheat accessions 10-A and B39. Twenty-four quantitative trait loci (QTLs) for TSPN and 18 QTLs for FSPN were localized based on the phenotype in 10 environments in 2019–2021. Two major QTLs, QTSPN/QFSPN.sicau-2D.4 (34.43–47.43 Mb) and QTSPN/QFSPN.sicau-2D.5(32.97–34.43 Mb), explained 13.97%–45.90% of phenotypic variation. Linked kompetitive allele-specific PCR (KASP) markers further validated these two QTLs and revealed that QTSPN.sicau-2D.4 had less effect on TSPN than QTSPN.sicau-2D.5 in 10-A×BE89 (134 RILs) and 10-A×Chuannong 16 (192 RILs) populations, and one population of Sichuan wheat (233 accessions). The alleles combination haplotype 3 with the allele from 10-A of QTSPN/QFSPN.sicau-2D.5 and the allele from B39 of QTSPN.sicau-2D.4 resulted in the highest number of spikelets. In contrast, the allele from B39 for both loci resulted in the lowest number of spikelets. Using bulk-segregant analysis–exon capture sequencing, six SNP hot spots that included 31 candidate genes were identified in the two QTLs. We identified Ppd-D1a from B39 and Ppd-D1d from 10-A and further analyzed Ppd-D1 variation in wheat. These results identified loci and molecular markers with potential utility for wheat breeding and laid a foundation for further fine mapping and cloning of the two loci.
Keywords
