Evolution of naturally arising SARS-CoV-2 defective interfering particles

Samer Girgis; Zaikun Xu; Spyros Oikonomopoulos; Alla D. Fedorova; Egor P. Tchesnokov; Calvin J. Gordon; T. Martin Schmeing; Matthias Götte; Nahum Sonenberg; Pavel V. Baranov; Jiannis Ragoussis; Tom C. Hobman; Jerry Pelletier

doi:10.1038/s42003-022-04058-5

Communications Biology (Oct 2022)

Evolution of naturally arising SARS-CoV-2 defective interfering particles

Samer Girgis,
Zaikun Xu,
Spyros Oikonomopoulos,
Alla D. Fedorova,
Egor P. Tchesnokov,
Calvin J. Gordon,
T. Martin Schmeing,
Matthias Götte,
Nahum Sonenberg,
Pavel V. Baranov,
Jiannis Ragoussis,
Tom C. Hobman,
Jerry Pelletier

Affiliations

Samer Girgis: Department of Biochemistry, McGill University
Zaikun Xu: Department of Cell Biology, U Alberta
Spyros Oikonomopoulos: McGill Genome Centre, Department of Human Genetics, McGill University
Alla D. Fedorova: School of Biochemistry and Cell Biology, University College Cork
Egor P. Tchesnokov: Department of Medical Microbiology and Immunology, University of Alberta
Calvin J. Gordon: Department of Medical Microbiology and Immunology, University of Alberta
T. Martin Schmeing: Department of Biochemistry, McGill University
Matthias Götte: Department of Medical Microbiology and Immunology, University of Alberta
Nahum Sonenberg: Department of Biochemistry, McGill University
Pavel V. Baranov: School of Biochemistry and Cell Biology, University College Cork
Jiannis Ragoussis: McGill Genome Centre, Department of Human Genetics, McGill University
Tom C. Hobman: Department of Cell Biology, U Alberta
Jerry Pelletier: Department of Biochemistry, McGill University

DOI: https://doi.org/10.1038/s42003-022-04058-5
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 12

Abstract

Read online

Genomes from defective interfering (DI) particles following serial passaging of SARS-CoV-2 reveal a fusion protein that attenuates viral replication. Synthetic, recombinant DI genomes are designed to interfere with SARS-CoV-2 replication.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal