Protocol for an open-source system to integrate calcium imaging, pupillometry, and locomotion-estimated tracking in head-fixed mice

Maria M. Ortiz-Juza; Jovan Tormes-Vaquerano; Sophia M. Hegel; Vincent R. Curtis; Rizk A. Alghorazi; Noah W. Miller; Ellora M. McTaggart; Nicolas C. Pégard; Jose Rodriguez-Romaguera

STAR Protocols (Dec 2024)

Protocol for an open-source system to integrate calcium imaging, pupillometry, and locomotion-estimated tracking in head-fixed mice

Maria M. Ortiz-Juza,
Jovan Tormes-Vaquerano,
Sophia M. Hegel,
Vincent R. Curtis,
Rizk A. Alghorazi,
Noah W. Miller,
Ellora M. McTaggart,
Nicolas C. Pégard,
Jose Rodriguez-Romaguera

Affiliations

Maria M. Ortiz-Juza: Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Jovan Tormes-Vaquerano: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Sophia M. Hegel: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Vincent R. Curtis: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Rizk A. Alghorazi: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Noah W. Miller: Neuroscience Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Ellora M. McTaggart: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA
Nicolas C. Pégard: Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Carolina Stress Initiative, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Corresponding author
Jose Rodriguez-Romaguera: Department of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Neuroscience Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Applied Physical Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Carolina Stress Initiative, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Carolina Institute for Development Disorders, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27514, USA; Corresponding author

Journal volume & issue: Vol. 5, no. 4
p. 103331

Abstract

Read online

Summary: A wide selection of behavioral assays in systems neuroscience relies on head-fixation protocols to integrate in vivo multi-photon imaging approaches. For this, simultaneous pupillometry and locomotion tracking in head-fixed mice are used to measure behavioral responses and identify neural correlates. Here, we present an open-source protocol for assembling a complete head-fixation system that integrates pupillometry and locomotion-estimated tracking with multi-photon calcium imaging. We include detailed procedures for head-fixation and for data collection. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

About the journal

Abstract

Keywords