Cell-specific mitochondria affinity purification (CS-MAP) from Caenorhabditis elegans

Abstract

Summary: Cell-Specific Mitochondria Affinity Purification (CS-MAP) enables isolation and purification of intact mitochondria from individual cell types of Caenorhabditis elegans. The approach is based on the cell-specific expression of a recombinant hemagglutinin (HA)-tag fused to the TOMM-20 protein that decorates the surface of mitochondria, thereby allowing their immunomagnetic purification. This protocol describes the CS-MAP procedure performed on large populations of animals. The purified mitochondria are suitable for subsequent nucleic acid, protein, and functional analyses.For complete details on the use and execution of this protocol, please refer to Ahier et al. (2018, 2021).

Keywords

• Cell Biology
• Cell separation/fractionation
• Metabolism
• Model Organisms
• Molecular Biology
• Protein Biochemistry