Comparative Study on Biosynthesizing Selenium Nanoparticles by Gum Arabic and Poly Anionic Cellulose to Prevent Radiation-Induced Death in Chinese Hamster Ovary (CHO) Cells

Abstract

Purpose: In premenopausal women, abdominopelvic radiotherapy may have a direct and profound effect on ovarian function. Stabilized selenium Nanoparticles (NPs) with some natural materials have been demonstrated to have high antioxidant activity and reduce radiation damage as a radioprotector. This study was done to compare the ability for the biosynthesis of selenium NPs by Gum Arabic (Se-GA) and Polyanionic Cellulose (Se-PAC) in the protection of Chinese Hamster Ovary (CHO) cells against radiation damage. Materials and Methods: First, Selenium Nanoparticles (SeNPs) were synthesized in the presence of GA and PAC. Then, CHO cells were cultured in-vitro and were randomly divided into six groups in different concentrations of Se-GA and Se-PAC to measure the biocompatibility of NPs. Finally, cells were treated with NPs and radiation (6MV, 2Gy), and the percentage of cell survival was determined by MTT assay. Both NPs with an average size of 20-30 nm and an absorption absorbance peak at about 300 nm using Ultraviolet-Visible (UV–Vis) spectroscopy. Results: According to the parametric t-test analysis, Se-GA nanoparticles with a concentration higher than 0.4 ppm significantly increased the radioprotective effect on CHO cells compared to the control group (P0.05). Conclusion: Se-GA nanoparticles have antioxidant properties, and the radiation protection properties of Se-GA nanoparticles are significantly higher than control. Consequently, Se-GA nanoparticles showed promising results and may be able to play the role of a radioprotector.

Keywords

• Radiotherapy
• Radiation Protection
• Gum Arabic
• Nanoparticles
• Ovary