An Investigation on Gene Regions Related to Some Economic Traits in Holstein and Brown Swiss Cattle Breeds by Using PCR-RFLP Technique

Abstract

In this study, the polymorphisms in calpain (CAPN1), estrogen receptor α (ERα), prolactin (PRL), and myostatin (MSTN) genes thought to be related to some economical traits were investigated in 136 heads from Holstein and Brown Swiss cattle breeds reared in Karacabey town of Bursa by using PCR-RFLP method. While MSTN locus was found as monomorphic, 2 alleles of each CAPN1, ERα and PRL genes were determined in both breeds. While 3 genotypes (CC, CT, and TT) were determined at CAPN1 locus of both breeds, 2 genotypes (AG and GG) and 3 genotypes (AA, AG, and GG) Juszczukwere Determined at PRL locus of Holstein and Brown Swiss breeds, respectively. Two genotypes (AG and GG) were detected at ERα locus in both breeds. Both two populations were found at Hardy-Weinberg equilibrium except for PRL locus in Brown Swiss population.

Keywords

• cattle
• polymorphism
• capn1
• capn1
• erα1
• prl ve mstn
• polimorfizm
• sığır