The impact of pH on cytotoxic effects of three root canal irrigants

Mohammad Ali Saghiri; Abbas Delvarani; Payman Mehrvarzfar; Mohsen Nikoo; Mehrdad Lotfi; Kasra Karamifar; Kamal Asgar; Sahar Dadvand

doi:10.1016/j.sdentj.2011.03.002

Saudi Dental Journal (Jul 2011)

The impact of pH on cytotoxic effects of three root canal irrigants

Mohammad Ali Saghiri,
Abbas Delvarani,
Payman Mehrvarzfar,
Mohsen Nikoo,
Mehrdad Lotfi,
Kasra Karamifar,
Kamal Asgar,
Sahar Dadvand

Affiliations

Mohammad Ali Saghiri: Department of Dental Material, Islamic Azad University (Dental Branch), Tehran, Iran; Corresponding author. Address: Department of Dental Material, Islamic Azad University (Dental Branch), P.O. Box 14665-1445, Tehran, Iran. Tel.: +98 2144475524; fax: +98 2144420250.
Abbas Delvarani: Department of Endodontics, Islamic Azad University (Dental Branch), Tehran, Iran
Payman Mehrvarzfar: Department of Endodontics, Islamic Azad University (Dental Branch), Tehran, Iran
Mohsen Nikoo: Department of Dental Material, Islamic Azad University (Dental Branch), Tehran, Iran
Mehrdad Lotfi: Research Center for Pharmaceutical Nanotechnology and Department of Endodontics, Dental Faculty, Tabriz University (Medical Sciences), Tabriz, Iran
Kasra Karamifar: Post graduate Department of Endodontics, Islamic Azad University (Dental Branch), Tehran, Iran
Kamal Asgar: Department of Dental and Biological Materials, University of Michigan, USA
Sahar Dadvand: Department of Dental Material, School of Dentistry, University of the Pacific, USA

DOI: https://doi.org/10.1016/j.sdentj.2011.03.002
Journal volume & issue: Vol. 23, no. 3
pp. 149 – 152

Abstract

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Aim: Cytotoxicity of root canal irrigants is important due to their close contact with host tissues. This study was to assess the possible impact of pH on cytotoxic effects of MTAD, 17% EDTA, and 2.6% NaOCl on the human gingival fibroblasts using MTT assay. Materials and methods: Human gingival fibroblasts were exposed to the irrigants and their viability was assessed after 1, 6, and 12 h. The pH of the medium was measured in each interval. Light absorption values were measured for each culture medium using Elisa Reader device. Results: NaOCl had significantly less cytotoxicity than EDTA and MTAD. Also irrigants cytotoxicity decreased in 12, 1, and 6 h, respectively. Conclusion: It seems that variation of the pH resulted in variation in the cytotoxicity of solutions; i.e., it follows the pattern of the pH variation. Keywords: Cytotoxicity, Ethylene diaminetetra acetic acid, MTAD, pH, Sodium hypochlorite

Published in Saudi Dental Journal

ISSN: 1013-9052 (Print); 2524-1702 (Online)
Publisher: Springer
Country of publisher: Switzerland
LCC subjects: Medicine: Dentistry
Website: https://link.springer.com/journal/44445

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