DUSP2 Deletion Inhibits Macrophage Migration by Inhibiting ERK Activation in Zebrafish
Yu-Jiao Li,
Xin-Liang Wang,
Ling-Yu Shi,
Zong-Yi Wang,
Zi-Ang Zhao,
Shu-Chao Ge,
Bing Hu
Affiliations
Yu-Jiao Li
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Xin-Liang Wang
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Ling-Yu Shi
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Zong-Yi Wang
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Zi-Ang Zhao
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Shu-Chao Ge
Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA
Bing Hu
Center for Advanced Interdisciplinary Science and Biomedicine of IHM, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China
Dual-specificity phosphatase 2 (DUSP2) regulates the activation of members of the mitogen-activated protein kinase (MAPK) family, which is involved in a variety of cellular processes including cell proliferation, differentiation, apoptosis, and migration. DUSP2 also regulates the expression of inflammatory mediators in macrophages; however, it remains unknown whether DUSP2 participates in macrophage migration. Here, using the tail fin injury model in zebrafish larvae, we found that the deletion of DUSP2 inhibited the expression of pro-inflammatory cytokines and macrophage chemokines. Moreover, live imaging results showed that the migration of macrophages to the injury site was inhibited after DUSP2 deletion. This inhibitory effect was mediated through the reduced activation of extracellular regulated protein kinases (ERK) in DUSP2 knockout zebrafish.
