Activity of Alanine aminopeptidase and its isoenzyms partially purified from the urine of chronic renal failure patients.

Abstract

The study was performed on 50 urine specimens of patients with chronic renal failure ,in addition to the 92 healthy specimens as a control group. The results of the study revealed that Alanine aminopeptidase (AAP) activity of chronic renal failure patients urine shows a highly significant increase (p<0.0001) compared to healthy subjects. Alanine aminopeptidase was purified from urine patients with chronic renal failure and healthy subjects by gel filtration using sephadex G-50, and two isoenzyms of (AAP) I, II were separated from patients urine using anion exchanger (DEAE-sephdexA-50).

Keywords

• activity
• alanine
• aminopeptidase
• its isoenzyms
• partially
• purified from
• urine
• chronic renal failure patients
• aap