Single-and Dual-species Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus sciuri on Catering Kitchen Surfaces in Health Establishments

Abstract

Introduction: Biofilms formed by Pseudomonas aeruginosa and Staphylococcus sciuri on the surfaces of food preparation tables present a significant risk to public health. This study aimed to investigate the adhesion and formation of single- and dual-species biofilms of P. aeruginosa and S. sciuri on granite and stainless steel surfaces. Materials and Methods: Bacterial strains were identified by polymerase chain reaction and sequencing methods. The plate count method and epifluorescence microscopy (EP) were used to assess adhesion and biofilm formation. Results: P. aeruginosa was more effective alone in adherence and biofilm formation than with S. sciuri. The EP dual-species biofilm analysis showed that biofilm cells heavily adhered to stainless steel 304 and granite, Rosa Porrino, whereas granite, Gris Pinhel, and stainless steel 316 provided less suitable growth matrices. Conclusion: The quantity of extracellular polymeric substances affecting the appearance of bacterial cells was of greater importance on granite than on stainless steel. The surface type has an effect on adhesion and biofilm formation in dual and single cases of biofilm formation.

Keywords

• adhesion
• biofilm
• p. aeruginosa
• s. sciuri