A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export

Dick J. H. van den Boomen; Agata Sienkiewicz; Ilana Berlin; Marlieke L. M. Jongsma; Daphne M. van Elsland; J. Paul Luzio; Jacques J. C. Neefjes; Paul J. Lehner

doi:10.1038/s41467-020-19032-0

Nature Communications (Nov 2020)

A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export

Dick J. H. van den Boomen,
Agata Sienkiewicz,
Ilana Berlin,
Marlieke L. M. Jongsma,
Daphne M. van Elsland,
J. Paul Luzio,
Jacques J. C. Neefjes,
Paul J. Lehner

Affiliations

Dick J. H. van den Boomen: Cambridge Institute of Therapeutic Immunology & Infectious Disease, University of Cambridge
Agata Sienkiewicz: Cambridge Institute of Therapeutic Immunology & Infectious Disease, University of Cambridge
Ilana Berlin: Leiden University Medical Centre, Leiden University
Marlieke L. M. Jongsma: Leiden University Medical Centre, Leiden University
Daphne M. van Elsland: Leiden University Medical Centre, Leiden University
J. Paul Luzio: Cambridge Institute for Medical Research, University of Cambridge
Jacques J. C. Neefjes: Leiden University Medical Centre, Leiden University
Paul J. Lehner: Cambridge Institute of Therapeutic Immunology & Infectious Disease, University of Cambridge

DOI: https://doi.org/10.1038/s41467-020-19032-0
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 18

Abstract

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Lysosomes play an important role in cellular LDL-cholesterol uptake. Here, the authors perform a genome-wide genetic screen for cholesterol regulators and identify C18orf8 as a conserved subunit of a trimeric Rab7 GEF that controls LDL trafficking and NPC1-dependent lysosomal cholesterol export.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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