OncoTargets and Therapy (Jul 2021)
Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
Abstract
Peng Wang,1,* Song Yang,1,* Shengbin Dai,1 Qingtao Ni,1 Huilan Liu,1 Lei Yu,1 Kaijin Lu,2 Gaohua Han,1 Junxing Huang1 1Department of Oncology, The Fifth Affiliated Hospital of Nantong University (Jiangsu Taizhou People’s Hospital), Taizhou, Jiangsu, 225300, People’s Republic of China; 2Department of Thoracic Surgery, The Fifth Affiliated Hospital of Nantong University, Jiangsu Taizhou People’s Hospital, Taizhou, Jiangsu, 225300, People’s Republic of China*These authors contributed equally to this workCorrespondence: Gaohua Han; Junxing HuangDepartment of Oncology, Jiangsu Taizhou People’s Hospital, The Fifth Affiliated Hospital of Nantong University (Jiangsu Taizhou People’s Hospital), 399 Hailing South Road, Taizhou, 225300, Jiangsu, People’s Republic of ChinaTel +8613852613099; +8613515153996Email [email protected]; [email protected]: The long noncoding RNA (lncRNA) GAPLINC, or gastric adenocarcinoma predictive long intergenic ncRNA, plays a carcinogenic role in a variety of different tumor types. There is limited information regarding the biological function of GAPLINC in the development of esophageal squamous cell carcinoma (ESCC).Methods: Surgical tissue samples of 40 patients undergoing ESCC radical surgery were collected, including ESCC tissues and corresponding adjacent normal tissues. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of lncRNA GAPLINC in the human ESCC cell line (TE11). The function role of LncRNA GAPLINC was detected after specific siRNA interference and overexpression in the TE11 cell line. The effects of LncRNA GAPLINC on ESCC cell proliferation, migration and invasion abilities were investigated by flow cytometry, using the Cell Counting Kit-8 (CCK-8), and by Transwell migration assays, respectively.Results: The expression of lncRNA GAPLINC in ESCC tissues was significantly higher than that in corresponding adjacent normal tissues (P< 0.05) and correlated with the degree of tumor differentiation (P< 0.05). Compared with human esophageal normal epithelial cell lines, the expression of LncRNA GAPLINC was significantly higher in the human ESCC cell line (P< 0.05). CCK-8 assays showed that LncRNA GAPLINC overexpression increased the growth rate of cells (P< 0.05). Transwell experiments showed that LncRNA GAPLINC overexpression increased the ability of cell migration and invasion compared to control cells (P< 0.05). Annexin V assay revealed that LncRNA GAPLINC silencing increased early stage apoptosis (P< 0. 05).Conclusion: Our results suggest that LncRNA GAPLINC may be used as a biomarker for the diagnosis and monitoring of ESCC, and may play an oncogenic role in ESCC.Keywords: esophageal squamous cell carcinoma, long non-coding RNA, GAPLINC, biomarker, expression
