Comparison of Two Diagnostic Methods for the Detection of Hepatitis B Virus Genotypes in the Slovak Republic
Mariia Logoida,
Pavol Kristian,
Andrea Schreiberova,
Patrícia Denisa Lenártová,
Veronika Bednárová,
Elena Hatalová,
Ivana Hockicková,
Sylvia Dražilová,
Peter Jarčuška,
Martin Janičko,
Štefan Porhinčák,
Monika Halánová
Affiliations
Mariia Logoida
Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Pavol Kristian
Department of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, Slovakia
Andrea Schreiberova
Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy, 041 81 Kosice, Slovakia
Patrícia Denisa Lenártová
Department of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, Slovakia
Veronika Bednárová
Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Elena Hatalová
Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Ivana Hockicková
Department of Infectology and Travel Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 041 90 Kosice, Slovakia
Sylvia Dražilová
2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Peter Jarčuška
2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Martin Janičko
2nd Department of Internal Medicine, Faculty of Medicine, Louis Pasteur University Hospital, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
Štefan Porhinčák
Centre of Applied Computer Science, P. J. Safarik University, 040 01 Kosice, Slovakia
Monika Halánová
Department of Epidemiology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 11 Kosice, Slovakia
The hepatitis B virus (HBV), belonging to the Hepadnaviridae family, is responsible for a global health concern still in the 21st century. The virus is divided into 10 genotypes, which differ in geographical distribution and in their effect on disease progression and transmission, susceptibility to mutations, and response to treatment. There are many methods for diagnostics of HBV and differentiating its genotypes. Various commercial kits based on real-time polymerase chain reaction (RT PCR) and hybridization available, as well as whole genome sequencing or the sequencing of only individual parts of the genomes. We compared a commercial kit AmpliSens HBV-genotype-FRT, based on RT PCR, with an adapted method of amplification of the surface genomic region combined with Sanger sequencing. In the examined samples we identified the A, B, C, D, and E genotypes. By PCR with Sanger sequencing, the genotypes were determined in all 103 samples, while by using the commercial kit we successfully genotyped only 95 samples, including combined genotypes, which we could not detect by sequencing.
