Generation of human induced pluripotent stem cell line (BCRTi007-A) from urinary cells of a patient with autosomal dominant polycystic kidney disease

Lilas Batool; Constanze Raab; Christien Madlen Beez; Andreas Kurtz; Maik Gollasch; Bella Rossbach

Stem Cell Research (Jun 2023)

Generation of human induced pluripotent stem cell line (BCRTi007-A) from urinary cells of a patient with autosomal dominant polycystic kidney disease

Lilas Batool,
Constanze Raab,
Christien Madlen Beez,
Andreas Kurtz,
Maik Gollasch,
Bella Rossbach

Affiliations

Lilas Batool: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
Constanze Raab: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
Christien Madlen Beez: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Deutsches Herzzentrum der Charité (DHZC), Department of Cardiothoracic and Vascular Surgery, Augustenburger Platz 1, 13353 Berlin, Germany
Andreas Kurtz: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Germany, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany
Maik Gollasch: Klinik und Poliklinik für Innere Medizin D – Geriatrie, Universitätsmedizin Greifswald, Ferdinand-Sauerbruch-Straße, 17489 Greifswald, Germany
Bella Rossbach: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Germany, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany; Corresponding author at: Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Germany, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany.

Journal volume & issue: Vol. 69
p. 103071

Abstract

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Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic disorder of adults, characterized by uncontrolled cysts formation that causes a gradual impairment of kidney function. We generated a human induced pluripotent stem cell (hiPSC) line from the urinary cells of a patient diagnosed with ADPKD using a non-integrating Epi5™ Episomal iPSC reprogramming strategy. Characterization of the cell line was performed regarding their undifferentiated status, differentiation potential, and quality control for karyotypic integrity, identity, and clearance of reprogramming vectors. The newly derived hiPSC line, namely BCRTi007-A, can be used in vitro for disease modeling of ADPKD as well as testing for novel therapeutic approaches.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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