Search for donors of powdery mildew resistance genes among seedless and table grape varieties

Abstract

Powdery mildew (Erysiphe necator) is one of the most common and economically significant diseases of grapes. Currently, the main method of controlling the disease is pesticide treatment. Breeding of resistant varieties is necessary to reduce chemical treatments. Currently, a number of grape resistance genes to powdery mildew and DNA markers for identification the allelic status of these genes are known. In a study to determine the presence of resistance loci Ren3 and Ren9, 25 genotypes of table grape varieties were analyzed, including 18 seedless varieties. DNA markers GF15-42, ScORGF15-02 were used to identify Ren3 gene, and CenGen6 – to identify Ren9 gene. DNA of cultivars Regent and Seyve Villard 12-375, which have resistance alleles, were used as positive controls. As a result of DNA marker analysis, it was determined that genotypes of table varieties Viking, Kodryanka, Moldova, Nadezhda AZOS, Original and seedless varieties Pamyati Smirnova, Kishmish Zaporozhskiy and Kishmish 342 carry loci of resistance to powdery mildew Ren3 and Ren9.