Adição de NaCI em soluções fixadoras - observações histológicas e histoquímicas<br>Addition of NaCl in fixative solutions - histological and histochemical observations

Abstract

Análise comparativa entre os resultados obtidos após fixação com formol neutro a 10% e com Bouin (convencional) e os resultados obtidos após fixação com essas mesmas soluções acrescidas de NaCI na concentração de 0,7% O orgão-objeto de análise foi a glândula salivar submandibular do rato e os resultados foram avaliados após colorações de H-E e tricrómico de Mallory e, ainda, mediante técnicas Histoquimicas para muco substâncias e proteínas.A comparative analysis of results obtained with different fixative solutions is presented. The fixatives used were neutral formaldehyde, conventional Bouin, and these same solutions containing 0.7% NaCl. The organ studied under each of these fixative conditions was the submandibular salivary gland of the rat, which was evaluated in parafin sections stained with HE and Mallory as well as by means of histochemical techniques for mucosubstances and proteins. The following conclusions are drawn: 1. An addition of 0.7% NaCl improves both the 10% formaldehyde and the Bouin fixatives; 2. The Bouin-NaCI fixative is good for general morphologic studies but neither for acini nor ductal granules; 3. For microscopic observations of acini and ductal granules the best fixative is the formaldehyde-NaCl; 4. The saline formaldehyde gives the most adequate fixation for histochemical techniques.