Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters

Yun JIANG; De-li WANG; Ming HAO; Jie ZHANG; Deng-cai LIU

Journal of Integrative Agriculture (Apr 2023)

Development and characterization of wheat–Aegilops kotschyi 1Uk(1A) substitution line with positive dough quality parameters

Yun JIANG,
De-li WANG,
Ming HAO,
Jie ZHANG,
Deng-cai LIU

Affiliations

Yun JIANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China; Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
De-li WANG: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Ming HAO: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
Jie ZHANG: Biotechnology and Nuclear Technology Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610061, P.R.China
Deng-cai LIU: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, P.R.China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China; Correspondence LIU Deng-cai, Tel/Fax: +86-28-86290004

Journal volume & issue: Vol. 22, no. 4
pp. 999 – 1008

Abstract

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Exploring novel high molecular weight glutenin subunits (HMW-GSs) from wild related species is a strategy to improve wheat processing quality. The objective of the present investigation was to identify the chromosomes of the wheat-alien introgression line N124, derived from the hybridization between Triticum aestivum with Aegilops kotschyi, and characterize the effects on quality-related traits. Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1Uk(1A) substitution line. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and reversed-phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae. kotschyi parent. PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were Ukx and Uky. Compared to the wheat parent, the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters. It can be served as a donor or bridge material for wheat quality improvement.

Published in Journal of Integrative Agriculture

ISSN: 2095-3119 (Print); 2352-3425 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.journals.elsevier.com/journal-of-integrative-agriculture

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