Comparative Transcriptomic Analysis of Archival Human Vestibular Schwannoma Tissue from Patients with and without Tinnitus

Krishna Bommakanti; Richard Seist; Phanidhar Kukutla; Murat Cetinbas; Shelley Batts; Ruslan I. Sadreyev; Anat Stemmer-Rachamimov; Gary J. Brenner; Konstantina M. Stankovic

doi:10.3390/jcm12072642

Journal of Clinical Medicine (Apr 2023)

Comparative Transcriptomic Analysis of Archival Human Vestibular Schwannoma Tissue from Patients with and without Tinnitus

Krishna Bommakanti,
Richard Seist,
Phanidhar Kukutla,
Murat Cetinbas,
Shelley Batts,
Ruslan I. Sadreyev,
Anat Stemmer-Rachamimov,
Gary J. Brenner,
Konstantina M. Stankovic

Affiliations

Krishna Bommakanti: Department of Otolaryngology–Head and Neck Surgery, Massachusetts Eye and Ear and Harvard Medical School, Boston, MA 02114, USA
Richard Seist: Department of Otolaryngology–Head and Neck Surgery, Massachusetts Eye and Ear and Harvard Medical School, Boston, MA 02114, USA
Phanidhar Kukutla: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Murat Cetinbas: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Shelley Batts: Department of Otolaryngology–Head and Neck Surgery, Stanford University School of Medicine, Stanford, CA 94305, USA
Ruslan I. Sadreyev: Department of Molecular Biology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Anat Stemmer-Rachamimov: Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Gary J. Brenner: Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Konstantina M. Stankovic: Department of Otolaryngology–Head and Neck Surgery, Massachusetts Eye and Ear and Harvard Medical School, Boston, MA 02114, USA

DOI: https://doi.org/10.3390/jcm12072642
Journal volume & issue: Vol. 12, no. 7
p. 2642

Abstract

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Vestibular schwannoma (VS) is an intracranial tumor that commonly presents with tinnitus and hearing loss. To uncover the molecular mechanisms underlying VS-associated tinnitus, we applied next-generation sequencing (Illumina HiSeq) to formalin-fixed paraffin-embedded archival VS samples from nine patients with tinnitus (VS-Tin) and seven patients without tinnitus (VS-NoTin). Bioinformatic analysis was used to detect differentially expressed genes (DEG; i.e., ≥two-fold change [FC]) while correcting for multiple comparisons. Using RNA-seq analysis, VS-Tin had significantly lower expression of GFAP (logFC = −3.04), APLNR (logFC = −2.95), PREX2 (logFC = −1.44), and PLVAP (logFC = −1.04; all p MMP9, CXCL9, IL16, PF4, ITK, and ACVR2A genes. Future studies are needed to examine the importance of these candidates and of inflammation in VS-associated tinnitus.

Published in Journal of Clinical Medicine

ISSN: 2077-0383 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine
Website: http://www.mdpi.com/journal/jcm

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