NADPH oxidase RBOHD contributes to autophagy and hypersensitive cell death during the plant defense response in Arabidopsis thaliana

H. B. Liu; X. D. Wang; Y. Y. Zhang; J. J. Dong; C. Ma; W. L. Chen

doi:10.1007/s10535-015-0519-9

Biologia Plantarum (Sep 2015)

NADPH oxidase RBOHD contributes to autophagy and hypersensitive cell death during the plant defense response in Arabidopsis thaliana

H. B. Liu,
X. D. Wang,
Y. Y. Zhang,
J. J. Dong,
C. Ma,
W. L. Chen

Affiliations

H. B. Liu: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China
X. D. Wang: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China
Y. Y. Zhang: Department of Plant Biology and Ecology, College of Life Sciences, Nankai University, Tianjin, P.R. China
J. J. Dong: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China
C. Ma: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China
W. L. Chen: MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, P.R. China

DOI: https://doi.org/10.1007/s10535-015-0519-9
Journal volume & issue: Vol. 59, no. 3
pp. 570 – 580

Abstract

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Autophagy has been implicated as a cellular protein degradation process that is used to recycle cytoplasmic components under biotic and abiotic stresses and so restrict programmed cell death (PCD). In this study, we report a novel regulatory mechanism by which NADPH oxidase respiratory burst oxidase homolog D (RBOHD) regulated pathogen-induced autophagy and hypersensitive (HR) cell death. We found that the Pseudomonas syringae pv tomato bacteria DC3000 expressing avrRps4 (Pst-avrRps4) induction of RBOHD-dependent reactive oxygen species (ROS) production promoted the onset of autophagy, whereas a pretreatment with an NADPH oxidase RBOHD inhibitor reversed this trend. The inhibitor significantly blocked pathogen-induced autophagosome formation and ROS increase. Moreover, we also show that in the wild-type and atrbohF mutant, Pst-avrRps4-induced cell death was limited, whereas in the case of the atrbohD mutant, the infection triggered a spreading-type necrosis. Our results demonstrate that the RBOHD-dependent ROS accumulation stimulated autophagosome formation and limited HR cell death.

Published in Biologia Plantarum

ISSN: 1573-8264 (Online)
Publisher: Institute of Experimental Botany of the Czech Academy of Sciences
Country of publisher: Czechia
LCC subjects: Science: Biology (General); Science: Botany: Plant ecology
Website: https://bp.ueb.cas.cz/

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