Na+/Ca2+ Exchanger Inhibitors Inhibit Neurite Outgrowth in PC12 Cells

Toru Oda; Toshiaki Kume; Yasuhiko Izumi; Kumatoshi Ishihara; Hachiro Sugmimoto; Akinori Akaike

Journal of Pharmacological Sciences (Jan 2011)

Na+/Ca2+ Exchanger Inhibitors Inhibit Neurite Outgrowth in PC12 Cells

Toru Oda,
Toshiaki Kume,
Yasuhiko Izumi,
Kumatoshi Ishihara,
Hachiro Sugmimoto,
Akinori Akaike

Affiliations

Toru Oda: Department of Pharmacology, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan; Laboratory of Neuropharmacology, Faculty of Pharmaceutical Sciences, Hiroshima International University, 5-1-1 Hirokoshingai, Kure, Hiroshima 737-0112, Japan
Toshiaki Kume: Department of Pharmacology, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan
Yasuhiko Izumi: Department of Pharmacology, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan
Kumatoshi Ishihara: Laboratory of Neuropharmacology, Faculty of Pharmaceutical Sciences, Hiroshima International University, 5-1-1 Hirokoshingai, Kure, Hiroshima 737-0112, Japan
Hachiro Sugmimoto: World-Leading Drug Discovery Research Center, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan
Akinori Akaike: Department of Pharmacology, 46-29 Yoshida-shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan; Corresponding author. [email protected]

Journal volume & issue: Vol. 116, no. 1
pp. 128 – 131

Abstract

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Abstract.: To elucidate the role of Na+/Ca2+ exchanger (NCX) in neurite outgrowth, we investigated the effects of NCX inhibitors on neurite outgrowth in PC12 cells. KB-R7943 and 3′,4′dichlorobenzamil, NCX inhibitors, inhibited the neurite outgrowth caused by nerve growth factor (NGF). NCX inhibitors inhibited the neurite outgrowth caused by dibutylyl cAMP, which rapidly reorganizes the cytoskeleton. KB-R7943 inhibited the neurite outgrowth caused by Y-27632, an inhibitor of Rho kinase (ROCK) that regulates actin. However, NCX inhibitors did not inhibit NGF-induced phosphorylation of extracellular signal-regulated kinase. These results suggest that NCX inhibitor affects downstream of the Rho–ROCK signal transduction pathways in neurite outgrowth. Keywords:: Na+/Ca2+ exchanger (NCX), neurite outgrowth, PC12

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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