Normalization of one-channel microarrays for identification of organisms

Abstract

Microarrays are widely used in gene expression analysis, but there are further areas they can be applied to, like e.g. the identification of organisms. To interpret and compare the results of microarray experiments it is necessary to standardize the data. In this context standardization is referred to as normalization. We present data derived from a microarray experiment aiming to identify different subtypes of the hepatitis C virus. Most of the methods developed to normalize microarray data are focused on gene expression analysis. Their use for the identification of organisms is restricted and needs adaption for the special requirements. Based on our data setting, we present several possibilities how to modify the existing methods and deal with the specific conditions.

Keywords

• normalization
• one-channel microarrays
• identification of organisms
• loess