Аграрная наука Евро-Северо-Востока (Jun 2024)

The use of targeted sequencing for genotyping sheep of the Dzhalginsky Merino breed

  • A. Yu. Krivoruchko,
  • A. A. Kanibolotskaya,
  • L. N. Skorykh,
  • O. N. Krivoruchko

DOI
https://doi.org/10.30766/2072-9081.2024.25.3.453-464
Journal volume & issue
Vol. 25, no. 3
pp. 453 – 464

Abstract

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Single Nucleotide polymorphisms (SNP) as the most important type of genetic variability are widely used to confirm the origin of farm animals and they play an important role in breeding and raising. Their most important advantage in the identification of SNP alleles is accuracy which makes it possible to determine the genotype precisely. AgriSeq (ThermoFisher (USA)) is a sequencing technology that can be used to purposefully amplify and re-sequence thousands of SNP targets in a single reaction. This method is specially adapted for animal husbandry and already contains ready-made panels for some types of domestic animals. However, in order to use them in sheep, it is necessary to pre-select loci suitable for genotyping by sequencing. The purpose of the work is to study the effectiveness of identifying and prevalence of loci from the proposed set of SNPs in the examination of new generations of sheep of the Dzhalginsky Merino breed. The material for the study was data from targeted sequencing of the genomes of Russian sheep breeds according to the formed set of loci in order to identify single-nucleotide polymorphisms. The proposed panel of loci, modified after validation on the second generation of animals, contains 352 substitutions suitable for genotyping by sequencing and 413 polymorphisms associated with meat productivity of animals. An assessment of the frequency of polymorphisms with a reliable relationship with meat productivity indicators between the groups born in 2021 and 2022 has shown that most substitutions almost do not differ in frequency between generations. The indicators of the frequency of occurrence of substitutions in the group of selected animals obtained as a result of research indicate that selected polymorphisms are located in loci that are not subjected to significant rearrangements for several generations and can be informative for quite a long time.

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