Characterization of the <i>TcCYPE2</i> Gene and Its Role in Regulating Trehalose Metabolism in Response to High CO<sub>2</sub> Stress
Yan-Fei Zhou,
Min Zhou,
Yuan-Yuan Wang,
Xin-Yi Jiang,
Pei Zhang,
Kang-Kang Xu,
Bin Tang,
Can Li
Affiliations
Yan-Fei Zhou
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
Min Zhou
Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China
Yuan-Yuan Wang
Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China
Xin-Yi Jiang
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
Pei Zhang
Jing Hengyi School of Education, Hangzhou Normal University, Hangzhou 311121, China
Kang-Kang Xu
Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China
Bin Tang
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China
Can Li
Key Laboratory of Surveillance and Management of Invasive Alien Species in Guizhou Education Department, Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biological and Environmental Engineering, Guiyang University, Guiyang 550005, China
Cytochrome P450 monooxygenase (CYP) is one of the three detoxification metabolic enzymes in insects, and is involved in the metabolism and transformation of endogenous substances as well as the activation and degradation of exogenous compounds. This study aims to reveal the molecular mechanism of CYP9E2 in Tribolium castaneum in adapting to high-CO2 stress. By predicting the sequence function of CYP9E2, analyzing the temporal and spatial expression profile of TcCYP9E2, and using RNAi to silence TcCYP9E2 combined with a high-CO2 stress treatment, we measured the carbohydrate content, trehalase activity, and gene expression levels in trehalose metabolism of T. castaneum. A bioinformatics analysis showed that the predicted molecular weight of the protein encoded by TcCYP9E2 is 60.15, the theoretical isoelectric point is 8.63, there is no signal peptide, and the protein is hydrophilic. An evolutionary tree analysis showed that TcCYP9E2 belongs to the CYP6 family and belongs to the CYP3 group; and the spatiotemporal expression profile results showed that TcCYP9E2 was highly expressed in the larvae midgut 48 h after injection of dsCYP9E2, with survival rates decreasing with the increase in CO2 concentration. Under the condition of 75% CO2, the contents of glycogen, glucose, ATP, and membrane-bound trehalase decreased significantly after the injection of dsCYP9E2. The expression of TRE-1, TRE-2, and GP in trehalose metabolism and energy pathways was significantly downregulated.
