Nature Communications (Feb 2021)

Incorporation of sensing modalities into de novo designed fluorescence-activating proteins

  • Jason C. Klima,
  • Lindsey A. Doyle,
  • Justin Daho Lee,
  • Michael Rappleye,
  • Lauren A. Gagnon,
  • Min Yen Lee,
  • Emilia P. Barros,
  • Anastassia A. Vorobieva,
  • Jiayi Dou,
  • Samantha Bremner,
  • Jacob S. Quon,
  • Cameron M. Chow,
  • Lauren Carter,
  • David L. Mack,
  • Rommie E. Amaro,
  • Joshua C. Vaughan,
  • Andre Berndt,
  • Barry L. Stoddard,
  • David Baker

DOI
https://doi.org/10.1038/s41467-020-18911-w
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 19

Abstract

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Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with increased photostability and smaller size, which bind a range of DFHBI chromophore variants.