Nature Communications (Feb 2021)
Incorporation of sensing modalities into de novo designed fluorescence-activating proteins
- Jason C. Klima,
- Lindsey A. Doyle,
- Justin Daho Lee,
- Michael Rappleye,
- Lauren A. Gagnon,
- Min Yen Lee,
- Emilia P. Barros,
- Anastassia A. Vorobieva,
- Jiayi Dou,
- Samantha Bremner,
- Jacob S. Quon,
- Cameron M. Chow,
- Lauren Carter,
- David L. Mack,
- Rommie E. Amaro,
- Joshua C. Vaughan,
- Andre Berndt,
- Barry L. Stoddard,
- David Baker
Affiliations
- Jason C. Klima
- Department of Biochemistry, University of Washington
- Lindsey A. Doyle
- Division of Basic Sciences, Fred Hutchinson Cancer Research Center
- Justin Daho Lee
- Molecular Engineering Ph.D. Program, University of Washington
- Michael Rappleye
- Institute for Stem Cell and Regenerative Medicine, University of Washington
- Lauren A. Gagnon
- Department of Chemistry, University of Washington
- Min Yen Lee
- Department of Chemistry, University of Washington
- Emilia P. Barros
- Department of Chemistry and Biochemistry, University of California, San Diego
- Anastassia A. Vorobieva
- Department of Biochemistry, University of Washington
- Jiayi Dou
- Department of Biochemistry, University of Washington
- Samantha Bremner
- Institute for Stem Cell and Regenerative Medicine, University of Washington
- Jacob S. Quon
- Institute for Protein Design, University of Washington
- Cameron M. Chow
- Department of Biochemistry, University of Washington
- Lauren Carter
- Department of Biochemistry, University of Washington
- David L. Mack
- Institute for Stem Cell and Regenerative Medicine, University of Washington
- Rommie E. Amaro
- Department of Chemistry and Biochemistry, University of California, San Diego
- Joshua C. Vaughan
- Department of Chemistry, University of Washington
- Andre Berndt
- Molecular Engineering Ph.D. Program, University of Washington
- Barry L. Stoddard
- Division of Basic Sciences, Fred Hutchinson Cancer Research Center
- David Baker
- Department of Biochemistry, University of Washington
- DOI
- https://doi.org/10.1038/s41467-020-18911-w
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 19
Abstract
Fluorescent protein reporters based on GFP exist, but have intrinsic disadvantages. Here the authors incorporate pH, Ca2+ and protein–protein interaction sensing modalities into de novo designed mini-fluorescence-activating proteins (mFAPs), with increased photostability and smaller size, which bind a range of DFHBI chromophore variants.
