Nature Communications (Mar 2022)

In vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterization

  • Céline Christiansen,
  • Deborah Maus,
  • Ellen Hoppenz,
  • Mateo Murillo-León,
  • Tobias Hoffmann,
  • Jana Scholz,
  • Florian Melerowicz,
  • Tobias Steinfeldt,
  • Frank Seeber,
  • Martin Blume

DOI
https://doi.org/10.1038/s41467-022-28730-w
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 15

Abstract

Read online

Bradyzoites are a quiescent form of Toxoplasma gondii enclosed in cysts during chronic infections. Here, Christiansen et al. develop a human myotube-based in vitro culture model of cysts that are infectious to mice and characterize their metabolism in comparison to fast replicating tachyzoites.