Nature Communications (Aug 2020)
Single-cell analysis uncovers fibroblast heterogeneity and criteria for fibroblast and mural cell identification and discrimination
- Lars Muhl,
- Guillem Genové,
- Stefanos Leptidis,
- Jianping Liu,
- Liqun He,
- Giuseppe Mocci,
- Ying Sun,
- Sonja Gustafsson,
- Byambajav Buyandelger,
- Indira V. Chivukula,
- Åsa Segerstolpe,
- Elisabeth Raschperger,
- Emil M. Hansson,
- Johan L. M. Björkegren,
- Xiao-Rong Peng,
- Michael Vanlandewijck,
- Urban Lendahl,
- Christer Betsholtz
Affiliations
- Lars Muhl
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Guillem Genové
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Stefanos Leptidis
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Jianping Liu
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Liqun He
- Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neuroinjury, Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City
- Giuseppe Mocci
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Ying Sun
- Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University
- Sonja Gustafsson
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Byambajav Buyandelger
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Indira V. Chivukula
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Åsa Segerstolpe
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Elisabeth Raschperger
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Emil M. Hansson
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Johan L. M. Björkegren
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Xiao-Rong Peng
- Bioscience Metabolism, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM) BioPharmaceuticals R&D, AstraZeneca
- Michael Vanlandewijck
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Urban Lendahl
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- Christer Betsholtz
- Karolinska Institutet/AstraZeneca Integrated Cardio Metabolic Centre
- DOI
- https://doi.org/10.1038/s41467-020-17740-1
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 18
Abstract
To define and distinguish fibroblasts from vascular mural cells have remained challenging. Here, using single-cell RNA sequencing and tissue imaging, the authors provide a molecular basis for cell type classification and reveal inter- and intra-organ diversity of these cell types.