Nature Communications (Mar 2016)

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

  • Ingrid Chamma,
  • Mathieu Letellier,
  • Corey Butler,
  • Béatrice Tessier,
  • Kok-Hong Lim,
  • Isabel Gauthereau,
  • Daniel Choquet,
  • Jean-Baptiste Sibarita,
  • Sheldon Park,
  • Matthieu Sainlos,
  • Olivier Thoumine

DOI
https://doi.org/10.1038/ncomms10773
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 15

Abstract

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The advent of fluorescence-based super-resolution microscopy has created a need for labeling strategies relying on small probes that minimally perturb protein function. Here the authors describe a labeling method that reduces protein tag and label sizes, allowing for accurate protein targeting and measurements of protein dynamics in tight cellular spaces.