Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang

Hongyang Zhang; Xiaoning Wang; Ping Hu; Wenjun Zhou; Min Zhang; Jia Liu; Yuerong Wang; Ping Liu; Guoan Luo

doi:10.3390/molecules21010126

Molecules (Jan 2016)

Serum Metabolomic Characterization of Liver Fibrosis in Rats and Anti-Fibrotic Effects of Yin-Chen-Hao-Tang

Hongyang Zhang,
Xiaoning Wang,
Ping Hu,
Wenjun Zhou,
Min Zhang,
Jia Liu,
Yuerong Wang,
Ping Liu,
Guoan Luo

Affiliations

Hongyang Zhang: School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Xiaoning Wang: E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Ping Hu: School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Wenjun Zhou: E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Min Zhang: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
Jia Liu: E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Yuerong Wang: School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China
Ping Liu: E-Institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Guoan Luo: School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

DOI: https://doi.org/10.3390/molecules21010126
Journal volume & issue: Vol. 21, no. 1
p. 126

Abstract

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Yin-Chen-Hao-Tang (YCHT) is a famous Chinese medicine formula which has long been used in clinical practice for treating various liver diseases, such as liver fibrosis. However, to date, the mechanism for its anti-fibrotic effects remains unclear. In this paper, an ultra-performance liquid chromatography-time-of-flight mass spectrometry (UPLC-TOF-MS)-based metabolomic study was performed to characterize dimethylnitrosamine (DMN)-induced liver fibrosis in rats and evaluate the therapeutic effects of YCHT. Partial least squares-discriminant analysis (PLS-DA) showed that the model group was well separated from the control group, whereas the YCHT-treated group exhibited a tendency to restore to the controls. Seven significantly changed fibrosis-related metabolites, including unsaturated fatty acids and lysophosphatidylcholines (Lyso-PCs), were identified. Moreover, statistical analysis demonstrated that YCHT treatment could reverse the levels of most metabolites close to the normal levels. These results, along with histological and biochemical examinations, indicate that YCHT has anti-fibrotic effects, which may be due to the suppression of oxidative stress and resulting lipid peroxidation involved in hepatic fibrogenesis. This study offers new opportunities to improve our understanding of liver fibrosis and the anti-fibrotic mechanisms of YCHT.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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